Recently there have been some questions regarding the use and conversion of files to the Bio-Rad PIC image format for use with the program Confocal Assistant. To clarify a couple of points: The Bio-Rad PIC file format utilizes the ability to store a Z series or time series as a single file. The PIC file itself consists of a 76 byte header followed by a pixel array of NX x NY x Npic bytes where NX and NY are X and Y pixel dimensions of the image and Npic is the number of images in the file. The format of the Bio-Rad header is available from the CoMOS reference section of the users manual. Further information about the Bio-Rad PIC file format itself, is available to Bio-Rad Confocal users upon request, from myself. Further user information on the Bio-Rad PIC file format and how to convert it to other formats can be found at the following web page maintained by Lance Ladic, University of BC Canada: http://www.cs.ubc.ca/spider/ladic/confocal.html and then select the Source Code & Tips icon. Andy Sowerby Product Manager Bio-Rad Microscopy Division Hemel Hempstead UK e-mail [log in to unmask] Telephone 44-1442-232552 xt 297 ______________________________ Reply Separator _________________________________ Subject: Re: converting TIFF to biorad .pic series Author: Confocal Microscopy List <[log in to unmask]> at Internet Date: 23/01/96 09:26 On Tue, 23 Jan 1996 14:41:36 +0100 Ralf Steinmeyer wrote: > > I have just tried the Program Confocal Assistant. As I understand it, to > work with series in Confocal Assistant a single .pic file has to contain > the whole series. Our ZEISS Confocal Microscope saves z-series as TIFF files > with one single image of the series in each file, so I think they have to > be connected to one .pic file. > > Does anyone know how to convert TIFF files taken as a z-series with a > ZEISS Confocal Microscope to a Biorad .pic file? Dear Ralph We have had a similar problem. The Biorad file (as I remember it -others please correct if I'm wrong) has a 512 byte header with lots of info about the picture etc. To translate the file from Zeiss tiff you will have to read the Zeiss header to get the resolution info etc. I have a Zeiss but I don't have the information on the Zeiss header. Perhaps someone else in the group has this info. in which case I would like it for future reference also. In any case, a special prgram will probably have to be written (by someone) to create a pic. file... Regards Mark Cannell