To Whom It May Concern, As a final (?) followup to the original poster who asked about converting a series of TIFF files generated by a Zeiss LSCM into a single Bio-Rad PIC file, I have now posted my crude workaround, complete with a simple MSDOS executable that will create a "dummy" Rio-Rad PIC file header that you can custom generate and add to an image stack. (all of this is assuming that you can convert your TIFF files to RAW files, using software I recommend.) I am not aware of how "customized" the Zeiss TIFF files are (i.e. with additional tags), but I would assume that you can treat them as "ordinary" grayscale TIFF files for the purpose of conversion to another image format? As an "added bonus" (!?) I have included some basic information about the Bio-Rad LUT (color Look Up Table) structure that some of you may find useful if using the Confocal Assistant software for customizing the colour of your confocal image stacks. All of this can be found on a Web page at my site: http://www.cs.ubc.ca/spider/ladic/fileform.html#HEADER Like I say, all of this is kind of crude, but it should work! I hope you find it useful! Regards, --Lance. -------------------- Lance Ladic Dept. of Physiology University of B.C. Vancouver, Canada --------------------