>I don't know if this is an appropriate group, but I was wondering if >anyone knows about propidium iodide's light sensitivity >characteristics. What wavelengths is it sensitive to? What is the >decay rate? etc... > >thanks, >Joe Hi Joe, We have used PI a lot for looking at fixed tissues. Use a standard rhodamine set (529ex/550em, works well on our confocal) and you should find that it is very bright and will not bleach significantly. Email me if you need specific details about conc. etc. Craig. ______________________________________________________________________________ Craig J. Daly Research Associate tel: 0141 330 6606 CRI Heart Failure fax: 0141 330 4100 IBLS email: [log in to unmask] University of Glasgow email: [log in to unmask] Glasgow G12 8QQ. Web Site http://130.209.48.91/pages/vascular.htm ____________________________________________________________________________ __