Dear Confocalists I am hoping to use a confocal to determine blood flow through an artery/vein with a goretex site. A small site of the artery is being removed and goretex tubing used to replace the dissected region. The artery is very close to the surface of the skin in the ear. I would like to know if anyone has techniques and/or suggestions of the most applicable dyes that may be useful for this experiment. It is important the dye is vital, as the experiment will be in vivo. It will be possible to block the artery for a short time (or time intervals) to stop blood flow for measurements?? Currently using an Optiscan LSCM. Thanks in advance TIM --------------------------------------------- The Microsearch Foundation of Australia Name: Tim Cushway E-mail: [log in to unmask] Date: 01/29/96 Time: 11:12:08 ---------------------------------------------