Hi Tim, Since I am unsure of your setup this is just a suggestion. We have found it possible to give very high concentrations (30mg/kg) of hoechst 33342 to in-vivo preps. You can then see the nuclei of the white blood cells flowing through the vessels. It also stains the nuclei of the vessels so you have a static image of the endothelial cell nuclei and a moving image of the white cells. All you need is a fluorescence 'scope with a UV block. However, I am sure it would be even better on a confocal. Craig. > >I am hoping to use a confocal to determine blood flow through an artery/vein >with a goretex site. A small site of the artery is being removed and goretex >tubing used to replace the dissected region. The artery is very close to the >surface of the skin in the ear. > >I would like to know if anyone has techniques and/or suggestions of the most >applicable dyes that may be useful for this experiment. It is important the >dye is vital, as the experiment will be in vivo. It will be possible to block >the artery for a short time (or time intervals) to stop blood flow for >measurements?? > >Currently using an Optiscan LSCM. > >Thanks in advance >TIM > >--------------------------------------------- >The Microsearch Foundation of Australia >Name: Tim Cushway >E-mail: [log in to unmask] > >Date: 01/29/96 >Time: 11:12:08 >--------------------------------------------- ______________________________________________________________________________ Craig J. Daly Research Associate tel: 0141 330 6606 CRI Heart Failure fax: 0141 330 4100 IBLS email: [log in to unmask] University of Glasgow email: [log in to unmask] Glasgow G12 8QQ. Web Site http://130.209.48.91/pages/vascular.htm ____________________________________________________________________________ __