At 12:15 PM 1/25/96 -0700, you wrote: >Hi, > We are about to try the fluorolink Cy5 kit. Have you used these? Are >they as simple to use as the instruction imply? Do you have any >suggestions before we start? > Thanks, We have used the fluorolink cy3 kit. It was easy to use as described. We started with synthetic substance P (11 amino acids) which showed a single peak by HPLC. We put the product through an HPLC and found there were many fluorescent peaks. We selected the peak corresponding to our expected molecular mass and had good success. Mass spec analysis confirmed that the dye had bound to only one sight on our molecule. I would recommend HPLC separation and analysis to be confident that the fluorescent mixture you use contains only your molecule of interest. Dr. Bridget Southwell Department of Anatomy and Cell Biology University of Melbourne Parkville Vic 3052 AUSTRALIA Tel: +61 3 9344-7646 Fax: +61 3 9347-5219