I'm a french student working with a confocal microscope. I'm having problems with getting images of fluorescence: there is an alleviation of the contrast and brightness while I get down into the stack. Does anyone have a solution to that problem or can someone give me references to articles dealing with image processing to correct the grey levels of my images? Many thanks. ======================== Francois ANGOT ======================== [log in to unmask] http://www.info.unicaen.fr/~fangot Groupe de Recherches en Informatique, Image et Instrumentation de Caen GREYC-ISMRA - 6 Bd Marechal Juin - 14050 CAEN Cedex - FRANCE ======================================================================