Bonjour Il y a plein de litterature a ce sujet: en particulier recemment dans Journal of Microscopy numero de Mars 1996, Cytometry Fevrier/Mars 1996. Il y a egalement les articles de Jean-Paul Rigaut dans Journal of Microscopy, Analytical quantitative cytology and Histology (Rigaut & Vassy, 1991,Anal.Quant. Cytol. Histol. 13:223-232). Voici egalement quelque references concernant notre travail. Parazza F., Humbert C., Usson Y., Method for 3D volumetric analysis of intranuclear fluorescence distribution in confocal microscopy, Computerized Medical Imaging and Graphics, 1993, 17 (3): 189-200. Usson Y., Parazza F., Jouk P.-S., Michalowicz G., Method for the study of the three-dimensional orientation of the nuclei of myocardial cells in fetal human heart by means of confocal scanning laser microscopy, Journal of Microscopy, 1994, 174: 101-110. Parazza F., Bertin E., Wozniak Z., Usson Y., Analysis of the spatial distribution of AgNOR proteins in cell nuclei using simultaneous confocal scanning laser fluorescence and transmitted light microscopy, Journal of Microscopy, 1995, 178 (3): 251-260. Wozniak Z.M., Usson Y., Parazza F., Champelovier P., Leroux D., Seigneurin D., Quantitative analysis of three-dimensional distribution of AgNOR proteins during interphase in leukemic cells, Cytometry, 1996, 24:14-26.