> > On Feb 10, 5:43pm, Rui Malho wrote: > > I agree, one noisy pixel could stop the accumulation, while the rest of the > image is significantly dimmer.. In order to be more quantitative, one can > check the pixel average variance (or standard deviation) over selected > parts of the two images. > The accumulate-to-peak function would be much more useful if it had smarter end conditions. For example, one could require that a minimum percentage of pixels reach a certain threshold. Or one could look at various moments of the histogram. And since there is so much time in the slow scan mode, one could run a median filter on the image (or a user-selected subset of the image) in order to reduce point noise, and then test. This is very similar to the problem of doing auto-exposure. You cannot solve every case, but you can do pretty well in most cases. Just another suggestion I would like to see implemented in the Biorad Lasersharp software... BTW, do the other (ie, Zeiss, Leica, Olympus, etc) confocal manufacturers do any of the above? --aryeh Aryeh Weiss | email: [log in to unmask] Department of Electronics | URL: http://optics.jct.ac.il/~aryeh Jerusalem College of Technology | phone: 972-2-6751146 POB 16031 | FAX: 972-2-6422075 Jerusalem, Israel | ham radio: 4X1PB/KA1PB