Y'all- the following question came from one of our users - any help/references would be greatly appreciated. In FACS analysis it is possible to quench autoflourescence in the FITC PMT by adding trypan blue to the FITC-labelled secondary antibody, without significant quenching any of the specific staining. We are having some problems with autoflourescence in lymphnode frozen sections from mink. Does anybody know if it is possible to quench autoflourescence in flourescence microscopy by trypan blue or by other means??? Thank you in advance...... Lu Lucia P. Barker, Ph.D. Microscopy Branch Rocky Mountain Laboratories 903 South 4th Street Hamilton, MT 59840 Telephone: (406) 363-9252 FAX: (406) 363-9371 Electronic Mail: [log in to unmask]