Dear Confocalists,
I am trying to determine the shapes of the cells grown in dense 3-D
cultures by means of membrane staining dye DII. The problem is that I
have to fix the cells before the observations. Both formaldehyde and
glutaraldehyde that I used for fixation seem to destroy cell
membranes so their fluorescence after staining is barely observable. I
would greatly appreciate any sugestions concerning fixation protocols
or solutions that would fix the cells not destroying the structure of
their membranes.
Thanks in advance,

Jarek Czyz

Dr. Jaroslaw Czyz
Department of Cell Biology
The Jan Zurzycki Institute of Molecular Biology
al. Mickiewicza 3
31-120 Krakow
Poland
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