>Date: Mon, 26 Apr 1999 19:07:38 -0400 >From: Patty Tam <[log in to unmask]> >Subject: Pseudocolour and NIH Image >To: [log in to unmask] > >Hi, I was wondering if someone could tell me how to make pseudocolour >images on NIH Image. I collect my images in black and white on the >confocal and when I open them in NIH Image I would like to colour them >red or green, depending on the label. > >Thanks for your help. > >Patty Tam >University of Toronto >Dept. Lab Medicine and Pathobiology Patty, Dispose all images; Open your two images (same size) in red, green order; Duplicate one of the images and Fill (black) to be blue slice; Windows to Stack(Stack menu; {to give 3 slice stack, You then have an RGB stack that can be saved} RGB to 8bit Indexed(Stack menu {will give 8-bit colour image that can be saved or will appear on opening RG(B) stack} Usually, I would use EnhanceContrast; ApplyLut; to automaticallty (or else manually) stretch contrast in each of the R,G images before making pusedocolour. The above could be easily automated via a simple macro. Greg Joss, School of Biological Sciences, Phone:(61)(2) 9850 8212 Fax: 9850 8174 Macquarie University, Email [log in to unmask] North Ryde, (Sydney,) NSW 2109, Australia