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Does anyone have information on analyzing the colocalization of two
fluorescently labeled antigens?  I have been trying to do this using Leica
LCS software (version 585) with little success.  This software can generate
a 2D plot of the fluorescent intensities and statistically analyze the
localization of pixels.  However, I can not get anything remotely resembling
a colocalization coefficient.  I have seen this published using Ultraview
LCI software.

I appreciate any input.

Jennifer Anolik, MD, PhD
Rheumatology/Immunology Unit
University of Rochester Medical Center