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Try DRAQ5.  actually, we just tried this last week for the first time
with mouse eggs-it worked great (Cy-5 range).  ~1:1000 (works with live
cells, takes a few minutes to get in),
http://www.biostatus.co.uk/draq5_features.html

Mike Mancini
Baylor College of Medicine

-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Louis Villeneuve
Sent: Thursday, February 06, 2003 3:59 AM
To: [log in to unmask]
Subject: DNA staining


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Hi everyone,

I am looking for staining the DNA of a mouse ovocyte.  It is for dual
labeling and my 1st fluorochrome is Cy3.  Propiduim iodine and Ethiduim
bromide are in the range of Cy3 so I can't use them.   I cannot use UV's
suitable fluorochrome because I don't have the laser for it.  So I am
looking for DNA markers emitting in the range of FiTC or Cy5 for dual
labeling.

Can't you help me...

Thanks

Louis Villeneuve
Louis Villeneuve
Technicien de recherche- microscope confocal

Institut de Cardiologie de Montreal- Centre de recherche
5000 Belanger Est
Montreal (Quebec)
H1T 1C8

tel: 514-376-3330-3511
fax: 514-376-1355
email: [log in to unmask]