Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Dear Cam,

Actually we have been having the unstability problem you decribe for  
two months.
To tell the complete story : just after we moved our two SP5 from one  
room to another, both of them started (on the same day) to crash every  
10-20 minute during scans.
We were running under the version 1.7.0 which was told not to be very  
stable, so we upgraded to version 1.8.0. Under this version the system  
was crashing, let's say, every hour. So we decided to downgrade to  
version 1.6.3 with which the system used to be much more stable.
Actually, the crash rate of our system is still something like one  
crash per hour, and we are still fighting to find the source of the  
problem, because it seems that it is not (only) a software version  
problem (otherwise it would not crash anymore with version 1.6.3, as  
it used to be).

All I can tell you is to check the following problem-causes we have  
been going through :

-disconnect your computer from any network and avoid having other  
software running paralelly to the LAS AF software : we have been told  
that antivirus updates during a scan may cause system to crash

-check if your system is on a stabilized power supply ; the scanhead  
may get crazy in case of fluctuations

-control the hardware tree of your system (or just send an error log  
to Leica service, they will control it) : in our case, the system was  
initialising looking for some non-descan system that we did not have

All these details may improve the stability of your system.
But we still did not manage to solve the problem completely from our  
side, so I would actually also be very interested in knowing if  
someone else is experiencing this kind of problem (and a solution  
would be very welcome...)

JC



"Nowell, Cameron" <[log in to unmask]> a écrit :

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Howdy List,
>                  We have a leica SP5 system with the leica super Z peizo
> stage that we recently had upgraded to version 1.8.0 of LAS AF. After
> the upgrade we ran a test expeirment over night (multiple stage
> positions and different z stacks) and it ran perfectly. Since then it
> has crashed about 15-20 times in the sapce of 3 days. We had a new super
> z stage installed at the same time as the upgrade (to correct some drift
> issues we were having). But i do not think the crashing is due to the
> super z stage because the software will crash when you use the zoom
> functions as well or use wide focus instead of galvo.
>
> Is anyone out there running version 1.8.0 of LAS AF and if so have you
> had similar issues. We have now downgraded the software back to 1.7.0
> and it seems to be running smoothly again. I just need to know if this
> is a global problem with ver 1.8.0 or it is something specific to our
> instrument. The leica technitian took 5 goes to get version 1.8
> installed as it kept failing at different parts of the firm ware upgrade
> process. Similarly when taking it back to ver 1.7 i ahd the same
> problems.
>
> On another note does anyone out there with a super z stage have it
> vibrate on them? Both stages we have had vibrate and emit a high pitched
> wine. If you poke it it will stop and everthing is fine. If it is
> vibrating when you are trying to capture an image then you don't get a
> good picture.
>
>
> Thanks
>
>
> Cam
>
>
> Cameron Nowell B. Sc (Hons)
> Microscopy Imaging and Research Core Facility
> Peter MacCallum Cancer Centre
> 7 St Andrews Place
> East Melbourne, Victoria 3002
> AUSTRALIA
> Phone: +61396561242
> Mobile: +61422882700
> Fax: +61396561411
>
>
>
> This email (including any attachments) may contain
> confidential and/or legally privileged information and is
> intended only to be read or used by the addressee.  If you
> are not the intended addressee, any use, distribution,
> disclosure or copying of this email is strictly
> prohibited.
> Confidentiality and legal privilege attached to this email
> (including any attachments) are not waived or lost by
> reason of its mistaken delivery to you.
> If you have received this email in error, please delete it
> and notify us immediately by telephone or email.  Peter
> MacCallum Cancer Centre provides no guarantee that this
> transmission is free of virus or that it has not been
> intercepted or altered and will not be liable for any delay
> in its receipt.
>