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Tim, the ResCalc app assumes a Nyquist sampling rate of 2.3x.
As for whether or not one should choose their image pixel size using this sampling factor of 2.3 or something else for confocal or widefield, I would refer the interested reader to the epic discussion on this topic back from 2007 (so as not to have it repeated again!):
http://lists.umn.edu/cgi-bin/wa?A2=ind0701&L=CONFOCALMICROSCOPY&D=0&P=15141
John Oreopoulos
On 2012-01-15, at 8:08 PM, Guy Cox wrote:
> There isn't any difference between widefield and confocal resolution in any normal fluorescence mode. Do you really need an app to work out 0.61 lambda/NA? And then to divide by 2.3 to get Nyquist? If you are in multiphoton it's a little more complicated since root 2 (1.414) comes in, but you can just use the formula 0.43 lambda / NA.
>
> Guy
>
> Optical Imaging Techniques in Cell Biology
> by Guy Cox CRC Press / Taylor & Francis
> http://www.guycox.com/optical.htm
> ______________________________________________
> Guy Cox, MA, DPhil(Oxon), Honorary Associate,
> Australian Centre for Microscopy & Microanalysis,
> Madsen Building F09, University of Sydney, NSW 2006
>
> Phone +61 2 9351 3176 Fax +61 2 9351 7682
> Mobile 0413 281 861
> ______________________________________________
> http://www.guycox.net
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Tim Feinstein
> Sent: Monday, 16 January 2012 4:54 AM
> To: [log in to unmask]
> Subject: Re: Light Lab by Carl Zeiss Microscopy for iOS devices
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi John,
>
> ResCalc is a nice app, but it seems to calculate res and nyquist for widefield microscopy only. Am I wrong about that? As it is I use the Nyquist calculator on the SVI site (no commercial interest) but I would love to have something off-line.
>
> Cheers,
>
>
> TIm Feinstein
>
> Sent from my iPad
>
> On Jan 15, 2012, at 10:40 AM, John Oreopoulos <[log in to unmask]> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Adrian, there is a fairly good iPhone application that does some of the things you mentioned. It's called ResCalc.
>>
>> John Oreopoulos
>> Research Assistant
>> Spectral Applied Research
>> Richmond Hill, Ontario
>> Canada
>> www.spectral.ca
>>
>> On 2012-01-15, at 1:03 AM, Adrian Smith <[log in to unmask]> wrote:
>>
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>> *****
>>>
>>> On 14/01/2012, at 9:01 PM, Johannes Amon wrote:
>>>
>>>> *****
>>>> To join, leave or search the confocal microscopy listserv, go to:
>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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>>>>
>>>> of course I can't tell you any specifics but at the moment we are evaluating a native port to
>>>> android ICS 4.0. at the end it always comes down to budget so it would help immensely if
>>>> you'd order some confocals right now ^^
>>>>
>>>> just joking, gonna keep you posted on this project
>>>
>>>
>>> I would love to find a decent resolution calculator that can be used on a smartphone/tablet (iOS preferably for me :)
>>>
>>> Add in various options for different digitisation approaches (PMT, cameras) with Nyquist shortcuts and it would be real winner…
>>>
>>> Regards,
>>>
>>> Adrian Smith
>>> Centenary Institute, Sydney, Australia
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