In message     Bob Summers List writes:
 
> The reason that most people do not dehydrate and clear immunostained
> specimens is that they are unwilling to take the time...and for thin
> stuff, like cultured cells, it probably doesn't make much difference.
 
--Bob--my impression has been that in tissue mounted in methyl salicylate, the
red fluorophores cyanine 3.18 and cyanine 5.18 work very well, but that
fluorescein photobleaches like mad.  Is this accurate in your experience?
 
Thanks--
 
Martin Wessendorf