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>
>
>I saw a post regarding lucifer yellow and Alexa Fluors for cell
>filling and I was hoping someone can give me some technical advice.
>I am attempting to record from interneurons in the hippocampus of
>adult rats using patch-clamp technique. I want to identify them
>morphologically using a fluorescent marker so I can visualize them
>on my fluorescent microscope that is part of my rig. Someone on the
>list had mentioned that they were filling neurons that they recorded
>with patch-clamp in just minutes. What type of fluorescent marker
>would you recomend and what concentration and preparation would you
>suggest? I would also appreciate if I could get some specifications
>for the pipet solution and any tips you may have as far as
>identifying interneurons in the hippocampus. Thank you in advance
>for your help, it will be greatly appreciated.
>
>
Harry Pantazopoulos
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