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Hi,

Some things just take time and you will need to spend the time to get a
true 3D image of your biofilms.   But you can shorten the time by
reducing the resolution, scanning one time instead of averaging multiple
scans, and using a lower magnification.  If you need a quality image,
you will just have to take the time.  I routinely do 200+ slices of
thicker biofilms and, yes, it takes a long time.

Deb Berglund
Montana State University

Wijnholds, Anita wrote:

>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Dear all,
>
>We are measuring the development of biofilms. The z-step is calculated
>according to the resolution of the lens. In first instance, we get only a
>few slices as the film is not so thick. But the film grows to more than 50
>micron and so we get a lot of slices with that resolution.The analysis of
>the stacks is going to take too much time.
>Can we reduce the amount of slices? What are criteria to do so?
>I hope you can give me some good thougts and ideas about this.
>
>Kind regards,
>
>Anita Wijnholds
>NIOO-CEME
>
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