Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

Hi Olaf,

Sorry, no 2p data. No real brightness on membrane protein data. At 
ISAC 2006 Roger mentioned a new, improved, mOrange (did not specify 
name). You can get up to three plasmids at a time from Roger. See 
http://www.tsienlab.ucsd.edu/SUBWEB%20-%20Samples/ and 
http://www.tsienlab.ucsd.edu/SUBWEB%20-%20Samples/Constructs.htm

Some listservites may find of interest my table of 800+ fluorescent 
protein data. The current version can be downloaded at 
http://home.earthlink.net/~pubspectra/McNamara2006FPaa-FluorescentProteinsDataTable.xls

Spectra of many FPs are in various files at 
http://home.earthlink.net/~pubspectra/

Best wishes,

George


At 04:04 AM 5/30/2006, you wrote:
>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Sorry, but the last mail accidentially went out before it was written.
>
>Hello all,
>
>I have a question concerning the 2P-properties of the new FPs
>
>mRFP1
>mHoneydew
>mBanana
>mOrange
>dTomato
>tdTomato
>mTangerine
>mStrawberry
>mCherry
>
>Published in
>
>Shaner NC, Steinbach PA, Tsien RY.
>A guide to choosing fluorescent proteins.
>Nat Methods. 2005 Dec;2(12):905-9.
>
>And
>
>Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien RY.
>Improved monomeric red, orange and yellow fluorescent proteins derived from
>Discosoma sp. red fluorescent protein.
>Nat Biotechnol. 2004 Dec;22(12):1567-72. Epub 2004 Nov 21
>
>Does anybody know the 2P excitation spectra (cross sections) ? Have they
>been measured?
>
>Does anybody have experience with how bright they actually come out / how
>well they are expressed when fused to membrane proteins? Can we expect them
>to be brighter than eGFP / eYFP in the membranes of our HeLa / Cos systems?
>
>If we would like to give it a try, what would be the easiest way to get
>them?
>
>Thanks for any help / tips / hints!
>
>Olaf
>
>--
>
>  ---------------------------------------------------------
>| Dr. Olaf Selchow                                        |
>| Central Imaging Facility: Microscopy & Image Analysis   |
>| University of Stuttgart                                 |
>| Institute of Cell Biology and Immunology - SFB 495      |
>| phone: +49 (0)711 685 68209                             |
>| http://www.uni-stuttgart.de/izi/microscopy/             |
>  ---------------------------------------------------------




George McNamara, Ph.D.
Division of Cancer Immunotherapeutics and Tumor Immunology
City of Hope National Medical Center
1500 E. Duarte Rd
Duarte, CA 91010
626-359-8111 ext 60035 office
[log in to unmask]
[log in to unmask]