Robert

I use both colocalization softwares and both work well. Bitplane
software needs a Unix System and it's more complicate, but it brings you
more information. In fact, I believe that it's the only system able to
do colocalization analysis between 3 channels (I don't know exactly if
Leica has recently improve it). Leica software is more friendly and it
works well for usual applications, but if you want to use it in all its
applications you need Leica files (if you have a Leica system is ok.
Metamorph  system works very well for colocalization quantitization
(only for two channels) and, as the Bitplane software, you can use it
with all Tif files.
Good luck


Susanna Castel

Robert Zucker wrote:
>
> Kees
> I know of two programs called Colocalization--One is by Bitplane (Swiss
> company) and the other version is released by Leica which is also called
> colocalization. They are not  the same product, Both products cost in the
> 5K range. A Unix system is necessary to run the Bitplane while the Leica
> runs on a NT system.
> Let me know if you find something else. Quatitation is an important aspect
> of confocal microscopy even though there is the school of thought that
> believes a "pretty picture" is all that is important.. Some of us have
> different expectations from opur data. Best wishes
> Bob
>
> Robert M. Zucker, PhD
> U.S. Environmental Protection Agency
> MD 72
> National Health and Environmental Effects Research Laboratory
> Research Triangle Park, North Carolina, 27711
> Tel: 919-541-1585; fax 919-541-4017
> e-mail: [log in to unmask]
>
>                     Kees Jalink <[log in to unmask]>
>                     Sent by: Confocal                 To:
>                     Microscopy List                   [log in to unmask]
>                     <[log in to unmask]        cc:
>                     FFALO.EDU>                        Subject:     colocalization
>
>                     02/28/01 09:24 AM
>                     Please respond to Confocal
>                     Microscopy List
>
> Dear all,
>
> we study the colocalization between simultaneously acquired images of 2
> different fluorophores, one red, one green. These dyes are more or less
> all over the cell, in a dark and bright spotted pattern. Rather than
> just taking 'representative' pictures that show what we hope to show,
> we'd like to express things a bit more quantitatively / statistically
> justified. What methods do people use to quantify colocalization?
>
> any comments wellcome.
> Thanks, Kees
>
> --
>
> Kees Jalink Ph.D.
> The Netherlands Cancer Institute, dept. of Cell Biology H1
> Plesmanlaan 121, 1066CX Amsterdam, the Netherlands
> 020-5121933 (tel office)/ ...1947 (tel lab) / ...1944 (fax) /
> [log in to unmask] (email)
> at home: Paulus Potterlaan 5, 2102CC Heemstede
> 0235-476047 / [log in to unmask]