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Zeiss uses the M27 thread in their new microscope stands. This is significantly larger than the old Zeiss mount, and a bit larger than Nikon. Zeiss makes adapters from the old to the new M27 mount, so it should be possible to get similar adapters from for Nikon objectives on the new Zeiss scopes. In fact, I think Zeiss even has adapters to mount the new larger objectives on the older scopes. However, all caveats mentioned by Chris remain valid. In particular, Nikon objectives are huge compared to Zeiss (Nikon 60x/1.4 Plan Apo is about 2 longer than the equivalent Zeiss.  

Julio Vazquez
Fred Hutchinson Cancer Research Center
Seattle, WA 98109

http://www.fhcrc.org/en.html

==



On Jul 8, 2013, at 11:47 AM, Chris Tully wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
> 
> Sripad,
> 
> Just for the purposes of full disclosure, I have previously (1998-2001)
> worked for a Nikon Microscopes dealer.  I now work for myself as an Image
> Analysis and Microscopy Consultant.
> 
> The Nikon TE2000-s microscope is part of the Eclipse series and uses the
> most recent revision of Nikon objectives.  Nikon Eclipse microscopes use
> the "new" CFI-60 objectives which have M25 threads, while Zeiss still uses
> the RMS (Royal Microscopy Society) standard. The M25 designation refers to
> the diameter of the thread in mm.  The RMS specification calls of a 0.8
> inch thread diameter (
> http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2818.1896.tb00905.x/abstract),
> which is significantly smaller.
> 
> While it is possible to buy (I think) or make an adapter that will fit an
> RMS threaded objective on to a scope that uses M25 threads, I doubt that
> the reverse is true.  An adapter that mounts a CFI-60 objective (or
> compatible mount) on an RMS scope would necessarily move the object being
> adapted at least 3-4 mm further away from the nose piece of the microscope,
> resulting in:
> 1. Unknown magnification (although this can be easily measured).
> 2. Parfocality issues.
> 3. Danger of banging the adapted objective into the slide after using a
> standard objective.
> 
> Also keep in mind the the CFI-60 objectives are not just bigger around than
> the RMS objectives they tend to be longer as well.
> 
> Chris Tully
> Microscopy and Image Analysis Consultant
> [log in to unmask]
> www.ImageIncyte.com
> [log in to unmask]
> 240-475-9753 (c)
> 
> [image: View my profile on LinkedIn]<http://www.linkedin.com/in/christully/>
> 
> 
> On Mon, Jul 8, 2013 at 2:22 PM, Sripad Ram <[log in to unmask]>wrote:
> 
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>> 
>> Hello,
>> We have a Zeiss axioObserver and we are trying to figure out whether we can
>> attach a Piezo nanopositioner that is currently installed on a Nikon
>> TE2000-s scope. The piezo is old and may not have the quicklock adaptor.
>> 
>> I was wondering if someone knew whether a Nikon to Zeiss adaptor is readily
>> available at someplace for this purpose?
>> 
>> 
>> Thanks.
>> 
>> Sripad
>>