Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

You do have to be careful with Hepes.  I've seen cells that bleb in it and
cells that are happy in it.  As with any media, you have to do controls.

Jennifer



On 6/1/07, Vickery Trinkaus-Randall <[log in to unmask]> wrote:
>
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> cells will bleb and you will see ruffling much more in HEPES than media
> - looks beautiful but it is stress!
>
> Jennifer Waters wrote:
>
> > Search the CONFOCAL archive at
> > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> >
> > Hi Jennifer,
> >
> > We purchased and like heaters from 20/20 Technology
> > (http://www.20-20tech.com/).  Also, try Gibco CO2 Independent media
> > for imaging.  Lots of cells seem to like it.  Alternatively, 25mM
> > Hepes added
> > to most medias will buffer without CO2.
> >
> > Best, Jennifer
> >
> > --
> > Jennifer Waters, Ph.D.
> > Director, Nikon Imaging Center at Harvard Medical School
>



-- 
Jennifer Waters, Ph.D.
Director, Nikon Imaging Center at Harvard Medical School