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mitochondria?
On Friday, May 28, 2004, at 11:19 AM, Mario Matijasic wrote:
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>
> I've thought about micoplasma contamination, made screens on the
> cultured
> cells and they were negative... As for the asumption of mitochondrial
> staining; wouldn't other cell lines also produce simmilar stain pattern
> then? I find the "granules" only in COS cell line... DRAQ5 staining of
> HEK
> 293, CHO, RBL cells in my experiments is strictly nuclear staining.
>
> Mario.
>
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]]
> On
> Behalf Of Tom Budd
> Sent: Friday, May 28, 2004 2:55 PM
> To: [log in to unmask]
> Subject: Re: COS cell nuclear stain
>
> Search the CONFOCAL archive at
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> mario
>
> are the COS cells in culture? i'm not familiar with them. if so, the
> cytoplasmic "granules" might be micoplasma contamination. we use
> DRAQ5 to
> screen some cell lines for this and other cytoplasmic critters.
>
> tbudd
>
> Mario Matijasic wrote:
>
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>> Dear all,
>>
>> I've been working with DRAQ5 nuclear stain for quite some time and it
>> is a wonderfull dye for DNA labeling. However, in my experiments with
>> COS cells,
>> DRAQ5 stains some cytoplasmatic granules as well as the nucleus (in
>> contrast to every other cell type I stained so far). The stained
>> granules are mobile in live cell experiments, but I have no idea what
>> they
> actually are...
>> Anybody had simmilar experiences?
>>
>> Thanks,
>> Mario.
>>
>> ---------------------
>> Mario Matijasic
>> Research Immunologist,
>> PLIVA Research Institute,
>> Prilaz baruna Filipovica 29
>> 10000 Zagreb, Croatia
>>
>> tel. +385 1 3721575,
>> fax. +385 1 3721957,
>> e-mail [log in to unmask]
>
> --
> Dr. T. Budd
> Professor and Chair of Biology
> St. Lawrence University
> Canton, NY 13617
> Phone = 315-229-5640
> Fax = 315-229-7429
> E-mail = [log in to unmask]
>
> This message is made of 100% recycled electrons!
>
>
---
Michael J. Herron, U of MN, Dept. of Entomology
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612-624-3688 (office) 612-625-5299 (FAX)
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