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I agree with Martin, first inform well IF and WHICH protocol you need.
Stereology has many approaches (cavalieri, fractionator, dissector,.) for
man different situations.  However, indeed more and more journals will ask
for this kind of analysis when you're eg working with neurons or gliacells
or plaques etc.  However, if it's a Zeiss microscope you're using for the
analysis, you might consider the KS-software (in future most probably also
available for AxioVision).  We had this software especially written for this
purpose by them, since we do many more types of analysis with the
KS-software, however, since that time I know other abs also showed interest.
Prices did not range up to several 10k's, since a motorized stage and z-axis
was already built in on our microscope.  The one thing I believe you should
look at is that it's user-friendly, since everything else is based on the
same mathematical algorithms.

Best and good luck!


Sven Terclavers

 

  _____  

From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Elaine Kunze
Sent: 05 February 2007 16:20
To: [log in to unmask]
Subject: [CONFOCAL] Unbiased Stereology

 

I have a user who wants to do unbiased stereology.  Are there programs out
there that do this?  Is there anywhere that I could find out more?  Sorry to
be so naive but I need a place to start.

Elaine Kunze
Flow Cytometry and Imaging
Huck Institutes of the Life Science
319 Life Sciences Building
Penn State University
University Park, PA 16802
814-863-2762