Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

I've thought about micoplasma contamination, made screens on the cultured
cells and they were negative... As for the asumption of mitochondrial
staining; wouldn't other cell lines also produce simmilar stain pattern
then? I find the "granules" only in COS cell line... DRAQ5 staining of HEK
293, CHO, RBL cells in my experiments is strictly nuclear staining.

Mario.



-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Tom Budd
Sent: Friday, May 28, 2004 2:55 PM
To: [log in to unmask]
Subject: Re: COS cell nuclear stain

Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal

mario

are the COS cells in culture? i'm not familiar with them.  if so, the
cytoplasmic "granules" might be micoplasma contamination.  we use DRAQ5 to
screen some cell lines for this and other cytoplasmic critters.

tbudd

Mario Matijasic wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
> Dear all,
>
> I've been working with DRAQ5 nuclear stain for quite some time and it
> is a wonderfull dye for DNA labeling. However, in my experiments with
> COS cells,
> DRAQ5 stains some cytoplasmatic granules as well as the nucleus (in
> contrast to every other cell type I stained so far). The stained
> granules are mobile in live cell experiments, but I have no idea what they
actually are...
> Anybody had simmilar experiences?
>
> Thanks,
> Mario.
>
> ---------------------
> Mario Matijasic
> Research Immunologist,
> PLIVA Research Institute,
> Prilaz baruna Filipovica 29
> 10000 Zagreb, Croatia
>
> tel. +385 1 3721575,
> fax. +385 1 3721957,
> e-mail [log in to unmask]

--
Dr. T. Budd
Professor and Chair of Biology
St. Lawrence University
Canton, NY 13617
Phone = 315-229-5640
Fax = 315-229-7429
E-mail = [log in to unmask]

This message is made of 100% recycled electrons!