We have noticed the same problem when we switched from COMOS 6.03 to
COMOS 7 on our Bio-Rad MRC 600. Some of the *.pic files are fine part way
into a data set and then are distorted("snowstorm effect", "rearranged
puzzle pieces") when we use confocal assistant 3.10. In some cases, the
data sets are not recognized at all. Other data sets collected at the
same time are fine. If you open them in Comos 6.03 they are fine.
 I would also be interested in finding out why this problems exists.
 
Patty Jansma                                    Tel:602-621-6671
[log in to unmask]
Arizona Research Labs Division of Neurobiology
University of Arizona
 
On Wed, 25 Sep 1996 [log in to unmask] wrote:
 
> We have encountered a problem with our Bio-Rad Comos 7 software for the
> MRC1000, which is quite puzzling to us. Increasingly we are analyzing our
> z-series with NIH Image or Confocal Assistant on a different computer.
> Loading the z-series with this programs we found that while some series can
> be opened without problems, some look like snowstorm pictures, in which no
> details of the original can be recognized. There is no obvious correlation
> to the size of the files. Furthermore the corrupt or the intact z-series
> could originate from the same confocal session and were recorded with
> identical settings. But the same corrupt files can be opened with the Comos
> software again without problems, so this is a problem only when working
> outside Comos i.e. with NIH Image or Confocal Assistant. It looks as if
> there has occured an alteration to the reading frame, but in Comos the
> files have the correct 768 x 512 pixels.
> Is there any reason or a solution known to this problem ?
> Any suggestions will be greatly appreciated.
> Thanks in advance
>
> Michael Kressel
> Institute of Anatomy
> University of Erlangen
> e-mail: [log in to unmask]
>