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Hi Elaine,

I haven't done this myself, but the first thing that comes to mind is filling up the pits with a fluorescent solution.  You could use FITC for this, mixed with some low melting point agar.

Good luck,

Steve
___________________________________________________
ir. Steve Koll
PhD Student
Ghent University
Lab of Biochemistry and Molecular Cytology
Coupure Links 653
9000 Gent
Belgium
Tel:++32/9/264 59 70
Fax:++32/9/264 62 19
http://allserv.rug.ac.be/~skoll

----- Oorspronkelijk bericht ----- 
  Van: Elaine Kunze 
  Aan: [log in to unmask] 
  Verzonden: donderdag 6 juni 2002 17:39
  Onderwerp: Metal pits


  Has anyone ever used confocal microscopy to measure the volume of pits in a metal surface?  I have tried reflective measurements, but can't tell where the pit begins or ends.  I know its a long shot but I figure if anyone knows, it will be this group.

  ****************************************************************************
          Elaine Kunze
          Flow Cytometry.....Image Analysis...
          Life Sciences Consortium
          http://www.lsc.psu.edu/stf/cqca/home.html
          8B Althouse Laboratory  (814-863-2762)
          Penn State University
          University Park, PA 16802




___________________________________________________
ir. Steve Koll
PhD Student
Ghent University
Lab of Biochemistry and Molecular Cytology
Coupure Links 653
9000 Gent
Belgium
Tel:++32/9/264 59 67
Fax:++32/9/264 62 19
http://allserv.rug.ac.be/~skoll