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Dear Sandrine,

I've seen this a couple of times with faulty pmt power supplies on Bio-Rad systems. As Mark suggests it could also be the laser. Do you see the same pattern when you collect images simultaneously with two detectors? If so probably the laser. Do you only see the problem with one laser or irrespective of which laser you use?

Good luck
Steve

Stephen H. Cody

On 23/10/2010, at 6:19 AM, Sandrine Pouvreau <[log in to unmask]> wrote:

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> 
> Hi all.
> We have a confocal microscope LSM 5 exciter (Zeiss) which is lately having 
> some troubles. In brief, the fluorescence intensity is changing during the 
> record, in a line –related pattern (in other word, the intensity changes happen 
> on one or several lines of the picture).
> Either we have a good resolution/high intensity picture, and the intensity is 
> dropping, or we have a poor resolution/low intensity picture, and the intensity 
> is abruptly increasing. This seems to (although we are not sure yet about this 
> part) increase when the confocal is on for a long time. 
> Did somebody experience the same phenomenon with a similar system? 
> The technician from Zeiss told us it could be a problem with one the 
> acquisition cards. However, our end of the year budget does not allow us to 
> change them. Is there any way we can try to at least minimize the problem?
> Thanks a lot.
> Regards
> Sandrine