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Dear Craig,
These two articles are very useful:
Gerald Donnert, Christian Eggeling & Stefan W Hell, "Major signal
increase in fluorescence microscopy through dark-state relaxation",
Nature Methods - 4, 81 - 86 (2007) doi:10.1038/nmeth986
In this paper, the photobleaching is minimized with dark state
relaxation (single photon excitation), which needs a low repetition
rate (<=1MHz).
Na Ji, Jeffrey C Magee & Eric Betzig, "High-speed, low-photodamage
nonlinear imaging using passive pulse splitters",
Nature Methods 5, 197 - 202 (2008)
In this paper, by increasing the repetition rate in two-photon
excitation, the effective excitation power is decreased, therefore a
lower photobleaching is obtained.
Thank you.
Sincerely,
Peng Xi
Ph. D. Associate Professor
Dept. of Biomedical Engineering, College of Engineering
Peking University, Beijing, China
Tel: +86 10-6276 7155
Email: [log in to unmask]
http://bme.pku.edu.cn/~xipeng
On Sat, Oct 30, 2010 at 2:30 AM, Craig Brideau <[log in to unmask]> wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi folks. I've been noticing a number of microscope companies have been
> offering 'white' tunable lasers with their confocals. Most of these systems
> appear to be pulse-laser driven supercontiuum-based light sources. My
> question for the list is will the pulsed excitation be more effective for
> even single photon fluorescence compared to conventional CW excitation? I'm
> thinking the relaxation time between pulses may help with photobleaching.
> Does anyone have any thoughts or experiences to share on the matter?
>
> Thanks,
>
> Craig
>
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