*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

TRACKING MOLECULES WITH LIGHT - Last chance to register
BIO-IMAGING WORKSHOP

WHERE: University of Western Sydney, Hawkesbury, NSW Australia
WHEN: 16 - 19 November, 2010. 

Registration and full program

www.uws.edu.au/3rd_advanced_bio-imaging_workshop 

The Workshop is for students and researchers at various levels of experience – with little prior training in confocal imaging or those interested in learning the more advanced techniques. A range of live samples will be used, including mammalian, invertebrate cells and tissues, plants and algae, biofilms and bacteria. 

You will be trained in the standard confocal techniques in 2D, 3D and 4D analyses, time-lapse imaging and interactive volume rendering. You will also be trained in the advanced techniques, focusing on protein localization and movement in cells; the analyses of their association with each other; concentrations, diffusion rates and lifetimes in sub-cellular compartments or in an entire cell. 


WORKSHOP FEES:

Students  AU$650 (GST inclusive)
All others  AU$850 (GST inclusive)

Accommodation: UWS College next to workshop venue AU $220 per week
Transportation and all light meals during workshop are covered by registration fees.

PACKAGE DEALS (INCLUDING FLIGHTS, ACCOMMODATION, TRANSPORT) ARE AVAILABLE. 
CONTACT Anya Salih [log in to unmask] for further information.

SCHOLRASHIPS for GRADUATE STUDENTS:

Becker & Hickl, who made fluorescence lifetime imaging methods available to laboratories world-wide, are providing 4 scholarships to graduate students to attend the workshop. Evidence of enrolment for a higher degree study must be provided at registration. Scholarships will be awarded on first come first served basis so reserve your place now. Flights and accommodation fees are not covered. Contact Workshop organiser Anya Salih for further information [log in to unmask], TEL. 61245701452.


ONE SCHOLARSHIP FOR EARLY CAREER RESEARCHER IS ALSO AVAILABLE.

 
Workshop presenters

.       Professor Takeharu Nagai, Laboratory for Nanosystems Physiology & Nikon Imaging Center, Hokkaido University Research Institute Electronic Science, Japan
.       Professor Enrico Gratton, Director Laboratory for Fluorescence Dynamics, University of California, Irvine
.       A/Professor Guy Cox, Australian Centre for Microscopy & Microanalysis, University of Sydney
.       Dr Renee Whan, Australian Centre for Microscopy & Microanalysis, University of Sydney
.       Professor Leann Tilley, Department of Biochemistry, La Trobe University
.       Dr Will Hughes, Director, Molecular Imaging Facility, Garvan Institute of Medical Research, University of Sydney
.       Dr Louise Cole, Advanced Microscopy Facility, Bosch Institute, Univ Sydney
.       Dr. Michelle Digman, Director Optical Biology Core Facility, University of California, Irvine
.       Dr Wolfgang Becker, Director Becker & Hickl GmbH, Berlin
.       Dr Mihail Matz, University of Texas, Austen
.       Dr Anya Salih, University of Western Sydney, Australia


Training sessions cover the following:

Cellular structural analysis in 2D, 3D and 4D 
Confocal imaging of live cells, dynamic tracking over time, imaging of cellular organelles and alterations during stress.

Multi-colour fluorescent proteins
Jelly-fish derived and coral GFP-type proteins fused to proteins of interest (e.g., mitochondrial, H2B histone, actin, tubulin, Golgi, membrane proteins); applications of novel Photoactive Fluorescent Proteins (EosFP, AmilRFP, kindling proteins) from reef corals to track cellular events. 

Confocal Spectral Imaging
Acquisition of microspectral data in 3D image stacks from samples with multiple fluorescent probes or from fluorescent coral tissues expressing a variety of GFP-type proteins.

Analysis of molecular movement and diffusion
Track proteins and other molecules in live cells. Measure protein femtoliter concentrations in sub-cellular compartments. Monitor mobility and binding using fluorescence correlation spectroscopy (FCS) etc.

Fluorescence Lifetime Imaging Microscopy (FLIM)
- a powerful tool to analyse spatial distribution of excited state lifetimes at every pixel of a sampled image: studied examples will include FRET-FLIM to study protein interactions (e.g., DNA-Protein) in cells and coral tissues, imaging of photoactivatable FPs, quenching of chlorophyll in symbiotic algae, etc. 


Workshop microscopes & companies

Leica TCS SP5 - two confocal microscopes with light and multiphoton lasers
Zeiss LSM 780 Confocal 
Nikon A1 Confocal 
Olymus FluoView FV1000
Ultra VIEW VoX spinning disc confocal microscope, 
Confocal FLIM system, Becker &  Hickl GmbH
Zeiss laser micro-dissection (Palm) microscope