Have never used any of the above; have used only propyl gallate (8%) in
glycerol, a recipe Steve Kempf gave me.  Works great.  Not good for living
cells, though, obviously!

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*       Anthony Moss                    voice  (334)844-9257            *
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On Fri, 5 Dec 1997, Susana Zanello wrote:

> Hello all,
>         In response to Christine's question about antifade reagents, I extensively
> use Prolong Antifade, and get, in general, very good results on cell
> culture preps (my usual dyes are Oregon Green and propidium iodide or
> relatives). However, let me take advantage of the subject being on and ask:
> has anyone had occasional trouble with it? Because I have. Sometimes, with
> no apparent reason (read as: "I don't know, but someone may"), the mounted
> slides turn yellowish as seen directly by the eye, and under the
> microscope, everything is fuzzy, almost as if the fluoresent dyes had
> "leaked" and were evenly distributed throughout the prep in and out of the
> cells. It looks really bad, and as I said, I cannot identify the cause and
> in this way I lose preps at random. HELP!
>
>         Thanks!
>                                 Susi
>
>
>