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We are trying to image ICG dye (indocyanine green, Ex. ~ 800nm) and getting a filter cube was simple. The difficulty is finding a light source that emits at a high enough frequency and making sure the detector, in our case, a CCD camera can see it. Manufacturers often put IR filters in both the camera and light source against potential heat damage so those have to be removed. 

I also found information about light source output from companies to be inconsistent as if they don't have too much experience with this range of wavelengths. Currently, we are dusting off an olde Xenon light source and will take a look.

Judy

Judy Trogadis
Bio-Imaging Coordinator
Keenan Research Centre, St. Michael's 
209 Victoria Street
Toronto M5B 1T8, Canada
office: 416-864-6060 ext. 77612
imaging facility:           ext. 77434
cell:        416-909-9878
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-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Kim Peifley
Sent: Thursday, December 08, 2011 4:06 PM
To: [log in to unmask]
Subject: Near IR Imaging

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Does anyone have suggestions on how to set up a microscope for imaging 
Near IR dyes? We would like to image Cy7, Cy7.5 and VisTag S750. We have 
Zeiss 510, 710 microscopes as well as Olympus FV1000 and TIRF3 microscopes.

Thanks for your help.

Kim Peifley


Kim Peifley (Contractor)  	 
Research Associate I				
Optial Microscopy and Analysis Laboratory	
http://atp.ncifcrf.gov				
SAIC-Frederick, Inc.			
National Cancer Institute at Frederick 
Post Office Box B
Frederick, MD 21702			
Phone: 301-846-6561			
Fax: 301-846-7672			
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