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Date: | Mon, 8 May 2006 10:14:19 -0500 |
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Dear all,
We’re looking to do some ratiometric calcium imaging on neuronal
cultures using a dual excitation flurophore (we don’t have the
capabilities to use dual emission). However, we also want to control
the excitability of our cultures using a light-gated cation channel
(Channel Rhodopsin 2), which excites around 488, close to the emission
of Fura-2 (~500nm). Thus, we’re afraid that emission of Fura-2 will
cause excitation of our channel (aberrantly) leading to a positive
feedback loop. Therefore, we would like to find a dye that uses dual
emission for ratiometric measurements, but is neither excited by, nor
emits around 488nm. Any thoughts?
Sincerely,
Jason P. Weick, PhD
University of Wisconsin-Madison
Waisman Center
1500 Highland ave.
Madison WI, 53705
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