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Dear all,

We’re looking to do some ratiometric calcium imaging on neuronal 
cultures using a dual excitation flurophore (we don’t have the 
capabilities to use dual emission). However, we also want to control 
the excitability of our cultures using a light-gated cation channel 
(Channel Rhodopsin 2), which excites around 488, close to the emission 
of Fura-2 (~500nm). Thus, we’re afraid that emission of Fura-2 will 
cause excitation of our channel (aberrantly) leading to a positive 
feedback loop. Therefore, we would like to find a dye that uses dual 
emission for ratiometric measurements, but is neither excited by, nor 
emits around 488nm. Any thoughts?

Sincerely,

Jason P. Weick, PhD
University of Wisconsin-Madison
Waisman Center
1500 Highland ave.
Madison WI, 53705