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Dear Nathalia,

I agree with Susanna. If  your  plan is to do mostly imaging of DAPI, 
then 405 nm laser will do the job.
405 can be also used for excitation of CFP and for photoactivation of pGFP.

Jan


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Jan Peychl, M.D., Ph.D.
Service Leader
Light Microscopy Facility
Max Planck Institute of Molecular Cell Biology and Genetics
Pfotenhauerstrasse 108
01307 Dresden
Germany

Tel.: +49 351  210 2502
Fax:  +49 351  210 2000




Susanna Castel wrote:

>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Dear Nathalia,
>
>If it is only for DAPI visualization you should purchase the blue diode
>(405). UV laser is much more expensive but it is necessary with no dubt  for
>Alexa Fluo 350, Indo-1, calcofluor, and others. Nevertheless,  DAPI and
>Hoescht can also be imaged with a 405 laser
>
>Good luck,
>
>Susanna
>
>
>Dr. Susanna Castel
>Confocal Microscopy and Cellular Micromanipulation Facility
>SCT- University of Barcelona
>Barcelona Science Park
>Josep Samitier, 1-5
>E-08028 Barcelona, Spain
>Ph. +34 93 403 46 55
>Fax. + 34 93 403 72 06
>http://www.sct.ub.es:802
>
>----- Original Message ----- 
>From: "Nathalia Holtzman" <[log in to unmask]>
>To: <[log in to unmask]>
>Sent: Tuesday, November 22, 2005 5:52 AM
>Subject: 405 vs true UV
>
>
>  
>
>>Search the CONFOCAL archive at
>>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>>
>>Hi,
>>
>>I am new here. Our department is about to buy a multi user confocal. We
>>    
>>
>are
>  
>
>>having a hard time deciding between a true UV line and the 405 laser. Our
>>budget is limited which makes the 405 appealing but we have a number of
>>    
>>
>user
>  
>
>>who insist we need the true UV for to visualize dapi. Is this true?
>>
>>
>>Also we have no other specific/current use for a true UV laser but there
>>    
>>
>is
>  
>
>>talk about needing one if we ever want to do cell ablations.  Is this
>>    
>>
>true?
>  
>
>>Are there any other reasons/ applications that make the UV better than the
>>405?
>>
>>Thanks in advance for your input.
>>
>>Nathalia Holtzman
>>Queens College, CUNY
>>    
>>