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Hi
At the end of your FRAP exp. you have a series of images when you go to gallery mode you can see all of them i.e. a pre-bleach, bleach and post bleach images.The recovery curve and the image series can be exported into  tiff fromat seperately and then saved so that you get both the images.
When you save an image with ROI it will show only that ROI in the image so you should be able to do your intensity measurememtns.Be careful while starting a fresh exp. you have to delete the exsisting ROI in the 'DEFINE REGION option of the Bleach Control and add a new one by giving it a diffrent name.If you do not delete you will get the previous ROI as well in your image.
The procedure for carrying out FRAP is mentioned in the help menu as 'FRAP GUIDE'provided you have the analysis software available i.e KINETIC'.
In short,
-Set the appropriate configurations as well as scan parameters.
-Go to edit bleach option and do appropriate settings.
-Define regions to be bleached.
-Set a time series by giving the time intervals and number of images.
-Say Start B.
You could just try the above. Best luck.

Vaishali Kailaje
Tata Memorial Centre, 
Advancd Centre for Treatment and Research in Cancer,
Kharghar,
Navi Mumbai,
India.


--- On Thu, 7/24/08, Yi Zheng <[log in to unmask]> wrote:

> From: Yi Zheng <[log in to unmask]>
> Subject: Question about LSM510 software
> To: [log in to unmask]
> Date: Thursday, July 24, 2008, 12:09 AM
> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> 
> Dear all,
> 
> I am practice FRAP on Zeiss LSM 510-META.
> 
> Right now I do know how to induce the bleach and record the
> recovery curve 
> of ROI(by using click "MeanROI" in time series
> control ). But everytime at the 
> end of recording, only the curves are saved. So my question
> is can I save the 
> series of image data as well as the  curve?
> 
> Another question is after induce bleach I need to restart
> the scanning and 
> draw the ROI to measure the mean intensity of them. I
> always worry about 
> that I might miss the ROI where bleaching happens. Does
> anyone here know 
> how to do it in a proper way? 
> 
> I really appreciate any help!
> 
> Thanks,
> 
> 
> Yi