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Dear Olaf,  do you see the problem in all PMT's? I had a similar problem
with a Leica TCS SP but it was mainly confined to the transmitted light
detector.  On some days it was better than on others.

John.

________________________

C. John Runions Ph.D.
Ottawa Health Research Institute
725 Parkdale Ave.
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-----Original Message-----
From: Olaf Selchow [mailto:[log in to unmask]]
Sent: Friday, August 08, 2003 8:12 AM
To: [log in to unmask]
Subject: Leica SP2 with 2p setup (Coherent Mira 900)


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Hello confocal colleagues...

I have a problem with our Leica SP2 system: The images show thin
dark, horizontal stripes. Sometimes more and sometimes less. I
couldn't figure out yet when the stripes are stronger and when not.
Sometimes they seem to have gone completely. It looks a little like a
phase-problem with the line scans. But I couldn't fix it by adjusting
the phase-correction.

One more hint I got is the following: The stripes were seen the first
time after the 2Photon system (IR laser Coherent Mira 900, EOM and
all the extra bits and pieces) has been added to the standard SP2.
Unfortunately this has been before I joined the lab. So I can not
confirm this myself. Before that the images were fine (that's what
the older colleagues here in the lab say).

Independent from the 2P system I thought that the stripes could come
from some noise on the electricity supply. We're a big institute and
I don't know what other equipment is used in the building... and as I
said I couldn't correlate it with any external parameter like
daytime, weekday or something else.

The images are sufficiently good quality to interpret. But they are
no good for publication....

Has anybody had such a problem before and/or have an idea about how
to solve it?
I'd like to avaid to call (and pay) the Leica service when it's maybe
just a very easy thing that I can do myself.

Thanks in advance for your help!

Olaf

--

  --------------------------------------------
| Dr. Olaf Selchow                           |
| SFB 495 Z1 - Central Microscopy Facility   |
| Institute for Cell Biology und Immunology  |
| University of Stuttgart                    |
| Allmandring 31, 70569 Stuttgart, Germany   |
| T: +49 711 685 8209, Fax: +49 711 685 7484 |
| email: [log in to unmask]    |
  --------------------------------------------