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Hi,
Has anyone have the experience of delivering 350nm laser in to a
microscope? I'm planning to use this line for autofluorescence
excitation with a Nikon microscope, I'll use the side-port to deliver
the excitation to construct a custom-made confocal setup, but I don't
know the absorption ratio. Currently I'm worrying about the absorption
from the reflective prism; if the absorption is very large I'll
consider to use the back port.
Thank you for any input!
Best,
Peng Xi
Associate Professor
Institute for Laser Medicine and Biophotonics
Shanghai Jiao Tong University
800 Dongchuan Rd.
Shanghai 200240, China
Tel: (86) 21-3420-4076
http://biophotonics.sjtu.edu.cn/