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Rosemary,

We recently used Imagene Green in Medicago roots to localize GUS 
expression in root nodules and root hairs. The root nodule expression 
was intensely labeled with controls showing absence of signal. Some 
empirical tweaking of incubation time may be needed. The labeling 
pattern via confocal microscopy basically showed a bright ring around 
the expressing cell walls with lower level cytoplasmic labeling.   In 
the root hair case, were were unable to see convincing signal using 
standard GUS expression and this was still the case with the Imagene 
Green. In this case, non-specific background prevailed. The standard PDF 
product description has two plant references at the end of the document 
that we used to get fixation and labeling conditions for plants which 
seemed to work for us.

Good Luck!

Best Regards, Kirk

Kirk J. Czymmek, Ph.D.
Associate Professor
Department of Biological Sciences
University of Delaware
Newark, DE 19713



Rosemary White wrote:

>Search the CONFOCAL archive at
>http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>
>Has anyone successfully used Imagene Green or Red to localise GUS expression
>in plants?  I just can't recall many publications with this, people mostly
>seem to use X-gluc.
>
>Thanks,
>Rosemary
>
>Dr. Rosemary White              [log in to unmask]
>Microscopy Centre                 ph.     61-2-6246 5475
>CSIRO Plant Industry            mob.  61-0402 835 973
>GPO Box 1600                        fax.     61-2-6246 5334
>Canberra, ACT 2601
>Australia
>
>  
>