How about some standard non-fluorescent nuclear stains like Geimsa or
Hematoxin (sp?).
 
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Paul Goodwin
Image Analysis Lab
FHCRC, Seattle, WA
 
On Wed, 2 Oct 1996, Michael J. Lyon wrote:
 
> I am looking for some help. I am trying to dissect out some very small
> <50 um blood vessels. The main problem is that I cannot see them once
> the blood has washed out.  I would like to stain the vessels with
> something that is compatible with immunohistochemical procedures using
> confocal microscopy.  I am concerned that the vessel staining will
> interfere with the fluorescence when viewed on the confocal either by
> scattering or autofluorescence.  Since alkaline phosphatase is present
> in most vessels and is still active after paraformaldehyde fixation,
> this should would work for the vessel stain.  I am using rats and most
> likely the method of Mayahara et al., Histochemie 11:88;1967.  Any
> advice would be appreciated.
>
> Thanks
>
> Mike
>