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Ciao Stefano,
I could suggest contact Dr Javier Adur (Lab Dra. María Fernanda Izaguirre) from Univ Entre Rios, Argentina, at [log in to unmask] ( http://www.bioingenieria.edu.ar/grupos/microscopia/MicroInicio.html). I know they have made such a Z- motorization in an Olympus microscope (sorry, no remember model) to perform deconvolution.

Regards,

José Pellegrino
Inst Exptl Physiol
CONICET - Natl Univ Rosario



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De: Confocal Microscopy List [[log in to unmask]] En nombre de Stefano Giovannardi [[log in to unmask]]
Enviado el: miércoles, 27 de junio de 2012 08:45 a.m.
Para: [log in to unmask]
Asunto: deconvolution

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Dear all,

I apologyze for this not real confocal question...
I am recently putting mi hands on a Olympus IX81 equipped for fluorescence image acquisition and I would like to move into deconvolution of wide field images.
I am asking If any of you tried this technique on this microscope, and the minimal optical/software configuration suggested (I have a light guide stable illumination, filter wheel for excitation filters, good CCD camera, vibration free environment, unfotunately not UPLANSAPO objectives at the moment)
I wonder also If the Z axis motorized movement is accurate enough for the purpose, the company says 10 nm steps...

Thanks, best regards
Stefano

Stefano Giovannardi PhD
DiSTA - BRD
Università dell'Insubria
via J.H. Dunant 3
21100 Varese Italy
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