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Dear all,

I apologyze for this not real confocal question...
I am recently putting mi hands on a Olympus IX81 equipped for fluorescence image acquisition and I would like to move into deconvolution of wide field images.
I am asking If any of you tried this technique on this microscope, and the minimal optical/software configuration suggested (I have a light guide stable illumination, filter wheel for excitation filters, good CCD camera, vibration free environment, unfotunately not UPLANSAPO objectives at the moment)
I wonder also If the Z axis motorized movement is accurate enough for the purpose, the company says 10 nm steps...

Thanks, best regards
Stefano

Stefano Giovannardi PhD
DiSTA - BRD
Università dell'Insubria
via J.H. Dunant 3
21100 Varese Italy
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