Dear Kenneth,
Your first problem could be solved in the same way as I did. I made
"permanent" files for the limited number of permanent users and one file
named "Guest" with the password "guest" for the incidental user, they
are instructed to remove images when finished.  Like you, we are trying
to keep our HD clean, and teaching our users to transfer their images to
their ZIPs, JAZes or to place their information in a file on our
network.

About your "second problem", we were dealing, thank the Lord, only once
with the same case. Ed Ballinger has changed, as you say "promptly" the
filter wheel motor and this disaster was over. We were waiting for him
for a couple of weeks, during this time I used my finger to place the
right filter in the right position.

I wish you good luck.

Kenneth A. Orndorff wrote:
>
>
>         I would like help with two problems we are  having with our Bio-Rad MRC-1024 confocal running LaserSharp 3.2 software.  Any input from others on the list would be greatly appreciated.
>          One problem involves corruption of the <lsrsharp.ini> file that contains information used for individual user folders.  We maintain separate directories for each user in the <ls_user> folder.  We carefully purge the image files to maintain plenty of room on the hard drive.  However, when we reach about 50 users we have had corruption of the <lsrsharp.ini> file. The software will crash and when we reboot there will only be manager and default user files accessible.
>         The second problem involves the filter wheel on PMT1.  The wheel will stop in a position partially blocking the beam path to the PMT or it may move to a totally incorrect filter position.  The software GUI indicates the proper postion but the filter wheel is physically not in the correct position.  This is most obvious when you see the reflected image of the illuminating beam creating an interference fringe pattern.
>         Bio-Rad has promptly replaced numerous electronic components on several occasions. These repairs  seem to cure the problem for a while ,  but  the problem slowly returns  over the course of several weeks use of the system. We would like to hear from anyone else who has seen these problems.  Thanks.
>
> Ken Orndorff
> H.C. Englert Cell Analysis Lab
> Dartmouth Medical School
> Borwell 383 West
> Lebanon, N.H.  03756
>
> Phone:  603-650-7661
> FAX:  603-650-6130
> Email:  [log in to unmask]

--
 Edward Monosov, Ph.D.
 Director
 The Cell Analysis Facility
 THE  BURNHAM  INSTITUTE
 Tel: (619) 646-3100
Office-(r.#5144):ext. x3206 (voice mail)
Multi-Photon Confocal Microscope(r.#5105),ext. x3206)
Fluorescent Microscopy Facility (r.#5121)
Electron Microscopy Laboratory.(r.#5123), ext. x3620

FAX  (619) 646-3196;
e-mail: [log in to unmask]