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Judy,

Give Draq-5 a try. We have had very good results when we have had to use
a nuclear probe in the high red.

                 -Ramin


-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On
Behalf Of Judy Trogadis
Sent: Monday, January 16, 2006 1:29 PM
To: [log in to unmask]
Subject: live nuclear dye

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Fellow Confocalists

I am looking for a nuclear counterstain for live cells which excites
around 633nm. Molecular Probes' Syto dyes (59-64), while they do excite
at that wavelength, also label mitochondria because of their positive
charge. 

We are already staining mitochondria with a red/green apoptotic marker
(red if live; green if apoptotic) so cannot change the wavelength of the
mitochondrial marker to green - are there any?

Thank you in advance for any suggestions
Judy

Judy Trogadis
Bio-Imaging Coordinator
St. Michael's Hospital, 7Queen
30 Bond St.
Toronto, ON M5B 1W8
Canada
ph:  416-864-6060  x6337
pager: 416-685-9219
fax: 416-864-6043
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