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I agree with Paul.  We just changed a Semrock for a Chroma filter cube 
set, which rectified a huge optical issue we were having that manifested 
as a large distinct region of non-TIR.  There was no visual signs that 
the Semrock had anything wrong with it, and remounting (gently) didn't 
affect the problem.
The new Chroma line filter set works perfectly as far as I can tell.

see here:
http://chroma.com/product/complete-filter-sets/laser-applications/dual--TRF59904-EM--ET-488-561nm-laser-Dual-Band-set-for-TIRF-applications

Neil

On 10/31/2013 4:18 PM, Paul Paroutis wrote:
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>
> Hi Ekaterina,
>
> We have an Olympus CellTIRF system and have observed that choosing a good
> quality dichroic can make or break your system. In fact, we went through 3
> 'identical' dichroics before settling on one that gave the best images and the
> lowest background/light scatter. You may want to try a few different dichroics and
> see if that helps...
>
> Thanks,
> Paul
>