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Hi, Ellen

Are you able to change the mounting material to 
something like glycerin which might match the RI 
of the mucus more closely but still be inert to 
the whole mount?  I don't know that this will 
work with living tissue, but it might be worth a 
try with the whole mount.  We played a similar 
trick on a materials science application when 
trying to look at very fine copper particles on a filter.

Also, try minimizing the pinhole on your 
confocal.  It should help reduce the scatter dramatically.

Good hunting!
Barbara Foster, President and Sr. Consultant

Microscopy/Microscopy Education
P: (972)924-5310
W: www.MicroscopyEducation.com

We are now scheduling courses through January 2013

At 10:27 PM 9/10/2012, Ellen T. Arena wrote:
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>
>I am hoping someone may have 
>suggestions/protocols for effectively removing 
>the mucus layer in intestinal tissue (especially 
>a problem in colonic tissues) for whole-mount 
>and/or live imaging.  I am having a great deal 
>of trouble imaging the tissue with 
>light-scattering of the mucus and difficulties 
>removing the mucus without disturbing the tissue 
>itself.  Does anyone have any suggestions?  It would be most helpful…
>
>Many thanks.
>
>Ellen
>
>
>
>------------------------------------------------------
>Ellen T. Arena, PhD
>Pasteur Foundation Postdoctoral Fellow
>Unité de Pathogénie Microbienne Moléculaire
>INSERM U786
>Institut Pasteur
>28 rue du Dr Roux
>F - 75724 PARIS Cédex 15
>France
>Tel: (33-0) 1 40 61 37 71
>Fax: (33-0) 1 45 68 89 53
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