Hello Anke:

Try the Hamamatsu C4878 cooler (you will need th holder as well).  It keeps the
MCP-PMT at -30 celsius.  Read the intructions carefully on operating the
cooler.

No financial interest.

Regards,
Sophie
____________________________________________________
Sophie M. K. Brunet, Ph. D.
Research Officer
Optical Spectroscopy, Laser Systems and Applications
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306-966-1719 (office)   306-966-1702 (fax)
____________________________________________________
Saskatchewan Structural Sciences Centre
University of Saskatchewan
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Saskatoon, Sk   S7N 5C9
____________________________________________________


Quoting Anke Bielicke <[log in to unmask]>:

> Hello everybody,
>
> I try to measure FRET with a FLIM system from Becker and Hickl containing a
> MCP detector from Hamamatsu (R3809U-50). This detector is very temperature
> sensitive (increase of dark counts with increasing temperature). Our room is
> air conditioned so the room temperature is constant.
>
> When I measure CFP over a couple of houres (I measure live cells and allways
> take a fresh probe) I observe a decrease in lifetime (0.4 ns in 5 hours).
>
> How can I avoid this problem??
> Has anybody else observed this?
> I think it could be the detector and that it is getting hot over the time.
> Could I cool it down somehow?
>
>
> Thanks for any helpful advise.
>
> Anke
>
>