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Hi Craig-

I'm pretty sure you want to be careful mixing the different oils-- they are
of different chemistry and might produce unpredictable results.  Maybe call
them and ask.  However, we always used the Cargille Laser Liquids (
http://www.cargille.com/laserliq.shtml) and the mixing trick there (I am
pretty sure you have to mix w/w but again call) worked quite well.  Most
likely, you'll want the 5610 series.  Regardless, note that all oils suffer
dispersion and wavelength and temperature differences from that specified
will cause them to have different  effective RIs when, for example, used at
37 deg C.

Cheers,

Jason

On Wed, Sep 3, 2008 at 10:25 PM, Craig Brideau <[log in to unmask]>wrote:

> Search the CONFOCAL archive at
> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
> Thanks for the tip!  I was looking on the Cargille website at the oil
> specs, and most of them are 1.52XX (including A and B) except for type FF,
> which is 1.48.  So theoretically you could mix the FF with A or B and get
> somewhere between 1.52 and 1.48 depending on the ratios.  Here's the table
> with the info:
> http://www.cargille.com/immeroilspecs.shtml
>
> Craig
>
>
>
> On Wed, Sep 3, 2008 at 2:36 PM, Barbara Foster <[log in to unmask]> wrote:
>
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>> Hi, Craig
>>
>> While mixing Type A and B will give you intermediatary thicknesses, it
>> will have little change on RI:  Type A and B are both 1.5150 for the
>> commonly used sodium D line.
>>
>> However, your concept is correct.  Cargille has several sets of test and
>> calibration liquids, and, by mixing them proportionately, you can, indeed,
>> achieve the intermediate refractive indices.  This practice has long been
>> used by chemical microscopists.
>>
>> For those of you interested in following through on this line of study,
>> Cargille used to have a great little reference book by Dr. R. D. Allen on
>> refractometry.  They also now have some basic but good info on their website
>> www.cargille.com). <http://www.cargille.com%29.%C2%A0/>
>>
>> Hope this was helpful,
>> *Barbara Foster, President
>>
>> Microscopy/Microscopy Education
>> *7101 Royal Glen Trail, Suite A
>> McKinney TX 75070
>> P: (972)924-5310
>> Skype: fostermme
>> W: www.MicroscopyEducation.com
>>
>>  <http://www.microscopyeducation.com/>*NEWS! Visit the NEW and IMPROVED
>> www.MicroscopyEducation.com <http://www.microscopyeducation.com/>! And
>> don't forget:  MME is now scheduling customized, on-site courses through Dec
>> 2008.  Call me for a free assessment and quote.
>>
>>
>>
>>
>>
>> *At 01:18 PM 9/3/2008, you wrote:
>>
>> Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>> I'm just using Cargille type A and B.  'A' for upright scopes and 'B' for
>> inverted (because it's thicker).  Apparently you can actually mix some of
>> these oils together to get intermediate thickness and index values, so it
>> may be possible to mix your own for an intermediate viscosity and index.  I
>> haven't tried this myself yet though.
>>
>> Craig
>>
>>
>> On Wed, Sep 3, 2008 at 9:56 AM, SUBSCRIBE CONFOCAL Neil M. Kad <
>> [log in to unmask]> wrote: Search the CONFOCAL archive at
>> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal
>> Hi all,
>> Just want to know what immersion oils people are using to ensure minimal
>> background.
>> I recently tried the Cargille FF, but because I'm using TIRF I saw an enormous
>> change in the image due to the RI difference compared to regular immersion
>> oils. So I'd like to stick to the usual 1.52 ish.
>> I'm working at 488nm for your information
>> Thanks for your help
>> Neil Kad University of Essex
>>
>>
>