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October 2009

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Subject:
Re: Optimum well medium volume for imaging in 96 wells plate
From:
TILL Photonics <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Tue, 13 Oct 2009 13:23:28 -0700
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I apologize, that was meant to go privately...
 


Date: Tue, 13 Oct 2009 16:07:53 -0400
From: [log in to unmask]
Subject: Optimum well medium volume for imaging in 96 wells plate
To: [log in to unmask]





Hi all,
 
We would like to get best  confocal images (on an Olympus FV300 confocal) of dim signal live cells cultured in a 96 well plate (glass bottom and dark edges).  Does some of you have found what is the best volume of medium to put per well in order to get a wide area of good focus for a couple of hours (2 to 3 hours)?  Any hints would be greatly appreciated.  Thanks a lot!  
 
Monique  		 	   		  
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